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PROCEDURE, FITTING AND ANALYSIS OF DETERMINING CONCENTRATIONS OF MULTIPLE ANALYZES IN A FLUID
PROCEDURE, FITTING AND ANALYSIS OF DETERMINING CONCENTRATIONS OF MULTIPLE ANALYZES IN A FLUID
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机译:测定流体中多种分析物浓度的程序,拟合和分析
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摘要
Determn. of two or more analytes in a liq. sample is effected by: (a) loading ligands specific for each analyte onto a support in discrete locations such that each location has not more than 0.1 V/K moles of ligand, where V (litres) is the vol. of the sample and K (l/mole) is the equilibrium constant of the ligand for the analyte; (b) contacting the support with the sample; and (c) measuring the amts. of bound analytes by a competitive or non-competitive assay method using a labelled reagent for each ligand, the reagent being capable of recognising the filled or unfilled binding sites on the ligand.
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机译:确定。液体中两种或多种分析物的混合通过以下方式影响样品:(a)在离散位置将对每种分析物具有特异性的配体加载到支持物上,以使每个位置具有不超过0.1 V / K摩尔的配体,其中V(升)为体积。样品的K(l / mol)是分析物配体的平衡常数; (b)使支持物与样品接触; (c)量度。通过使用竞争性或非竞争性分析方法对每个配体使用标记试剂分析结合的分析物,该试剂能够识别配体上的填充或未填充结合位点。
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