首页> 外国专利> FULL LENGTH TRANSCRIPT PROMOTER FROM FIGWORT MOSAIC VIRUS FOR STRONG AND UNIFORM EXPRESSION FOR CHIMERIC GENES INTO PLANT CELLS

FULL LENGTH TRANSCRIPT PROMOTER FROM FIGWORT MOSAIC VIRUS FOR STRONG AND UNIFORM EXPRESSION FOR CHIMERIC GENES INTO PLANT CELLS

机译:FIGWORT马赛克病毒的全长转录启动子,可将嵌合基因强而均匀地表达到植物细胞中

摘要

A full-length transcript promoter from figwort mosaic virus (FMV) is identified and its DNA sequence given. The promoter functions as a strong and uniform promoter for chimeric genes inserted into plant cells. This strong promoter function is exhibited by a histochemical assay in floral buds and by reproductive scores of transgenic plants including the promoter. The promoter preferably includes a 5 min leader sequence that may be from the FMV itself or from a heterologous source with respect to the promoter. The promoter is used in a plant cassette vector, a chimeric gene and in methods for transforming plant cells to obtain transgenic plants, plant cells or seeds incorporating the FMV promoter.
机译:鉴定了来自非花果花叶病毒(FMV)的全长转录启动子,并给出了其DNA序列。该启动子对于插入植物细胞中的嵌合基因起着强而均匀的启动子的作用。这种强的启动子功能通过花芽中的组织化学测定和包括启动子的转基因植物的繁殖分数表现出来。启动子优选包括5分钟的前导序列,其相对于启动子可以来自FMV本身或来自异源。该启动子用于植物盒载体,嵌合基因和转化植物细胞以获得掺入FMV启动子的转基因植物,植物细胞或种子的方法中。

著录项

  • 公开/公告号NZ235887A

    专利类型

  • 公开/公告日1993-02-25

    原文格式PDF

  • 申请/专利权人 MONSANTO CO;

    申请/专利号NZ19900235887

  • 发明设计人 ROGERS STEPHEN GARY;

    申请日1990-10-30

  • 分类号C12N15/54;C12N15/82;C12N15/84;C12N5/10;C07H21/04;A01H5/00;

  • 国家 NZ

  • 入库时间 2022-08-22 05:10:40

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