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METHOD OF IMPROVING YIELD OF DISULPHIDE-BONDED PROTEINS BY USING THIOL REAGENTS
METHOD OF IMPROVING YIELD OF DISULPHIDE-BONDED PROTEINS BY USING THIOL REAGENTS
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机译:硫醇试剂提高二硫键结合蛋白产量的方法
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摘要
The invention relates to a method for enhancing the development of the natural protein conformation on secretion of proteins with a disulphide bridge by a prokaryotic host organism which contains a recombinant DNA which codes for the secreted protein, in which the host organism is cultured in a suitable nutrient medium under suitable conditions for the expression of the recombinant DNA, which is characterised in that a nutrient medium containing 0.1 to 20 mmol/l of one or more thiol reagents is used.
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