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method for the production of determinants that are on the surface of filamentous capsid peptides capable of binding biotin, and filamentous and peptide determinants so obtainable.
method for the production of determinants that are on the surface of filamentous capsid peptides capable of binding biotin, and filamentous and peptide determinants so obtainable.
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机译:用于在能够结合生物素的丝状衣壳肽表面上产生决定簇的方法,以及由此获得的丝状和肽决定簇。
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摘要
A method for isolating filamentous phages capable of binding biotin, essentially characterized by the following operations: biotinylation of a protein used for the screening of a phage library, in which, on the surface of the capsid, are expressed oligopeptides coded by random sequence oligonucleotide inserts, introduced into a gene coding for a protein of the phage capsid using genetic manipulation techniques; screening the above library using the biotinylated protein; and isolating the phages capable of binding to biotin. Figure 1 shows, in the form of a histogram, the binding of biotinylated proteins to phages selected according to the method of the invention and immobilized on plastic. IMAGE
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