One-step immunoassay for the determination of antigen-specific antibody from one of the immunoglobulin classes A, M, D or E and a suitable agent.

摘要

The immunoassay entails a solid phase to which antibodies against the immunoglobulin class, or a fragment of an antibody of this type, are bound being contacted with an immunoglobulin-containing fluid, resulting in the immunoglobulin of this class being bound to this solid phase, and this solid phase being contacted with the antigen which optionally carries a label, and with a labelled antibody or a labelled fragment of an antibody against the antigen, if the antigen is unlabelled, and the amount of these antibodies which are specific for an antigen from one of the immunoglobulin classes being determined from the amount of label bound to the solid phase, and is characterised in that the solid phase is simultaneously in contact with the fluid containing the antibodies to be determined and with the optionally labelled antigen, and a substance which prevents immunoglobulin G binding to the solid phase, and, where appropriate, antigen binding to immunoglobulin G is added.