METHOD OF SELECTING AT LEAST ONE MUTATION SCREEN, ITS APPLICATION TO A METHOD FOR RAPID IDENTIFICATION OF ALLELES OF POLYMORPHOUS SYSTEMS AND DEVICE FOR IMPLEMENTATION THEREOF

摘要

2123789 9311262 PCTABS00022 Methods of selecting at least one mutation screen from a series of allele sequences of a polymorphous gene and for rapid identification of alleles of polymorphous genes, nucleotide probes obtained from the said mutation screens, placed specifically in data bank form, and a device for implementing the said method are disclosed. The method for identifying alleles consists of: (a) selecting all or part of a known consensus sequence of the said polymorphous gene; (b) creating a mutation matrix for the corresponding sequences of known alleles; (c) identifying indiscernable sequences by comparison in twos (alleles having the same mutation profile in the sequence selected in (a)) and excluding one of the members of the said pairs; (d) identifying and counting the obligatory mutations or allele marker mutations, i.e. those which are necessary and adequate for distinguishing between two alleles which are otherwise identical (set O of obligatory mutations); and (f) obtaining the said minimum mutation screen(s), comprising at least the obligatory mutations of step (e); then (g) selecting from the screen selected in step (f) of the said mutation screen selection process the most suitable mutation screen for preparing oligonucleotide probes suitable for use in differenciating all the alleles; (h) appropriately hybridizing an allele X to be identified with the oligonucleotide probes selected from the mutation screen(s) obtained in steps (a) to (g); and (i) identifying the allele X by detection of the said hybrid(s) which may have been formed in step (h).