Biosynthetic 2,5-diketocluconic acid reductase, processes, recombinant cells and expression vectors for its production and its use in preparing 2-keto-L-Gulonic acid

摘要

A protein having 2,5-diketogluconic acid (2,5-DKG) reductase activity is produced by recombinant host cells. This can be used to convert 2,5-DKG stereoselectively into 2-keto-L-gulonic acid (2-KLG) which is a useful intermediate in the production of ascorbic acid (vitamin C).