QUANTIFICATION OF BACTERIA USING A NUCLEIC ACID HYBRIDIZATION ASSAY

摘要

the invention relates to a method for the quantification of bacteria using a nucleic acid probe specific for bacteria, which is complementary to a region of highly protected single ribrosomique 16s rna (rrna) of bacteria.this probe allows rapid detection of 16s rrna in a sample, and by comparison with known standards, we can estimate the total bacterial count in the sample. the method is accurate and reproducible, and implemented at a temperature in the range of about 12 degrees and about 40 degrees.