METHOD OF UNKNOWN SEQUENCE DNA-FRAGMENT AMPLIFICATION LOCATED AT SOME DISTANCE FROM DNA-FRAGMENT WITH KNOWN SEQUENCE

摘要

Usage: molecular genetics, namely methods of propagation of DNA fragments of unknown DNA sequences from a particular region. SUMMARY OF THE INVENTION: DNA is carried out successive processing restrikta- Zami and doping, resulting in a fragment of DNA of unknown sequence, located in the native DNA at some distances from the DNA fragment of known sequence, It appears immediately adjacent to the DNA fragment of known sequence; Synthesis of oligonucleotide primers, carrying with them the use of amplification (multiplication) fragment of unknown DNA sequences 3 yl. SЈ