HYDROPHOBINS FROM EDIBLE FUNGI, GENES, NUCLEOTIDE SEQUENCES AND DNA-FRAGMENTS ENCODING FOR SAID HYDROPHOBINS, AND EXPRESSION THEREOF

摘要

The present invention relates to a hydrophobin, or a protein or polypeptide essentially corresponding thereto, derived from an edible fungus, preferably with GRAS status, more preferably from the genus Agaricus, such as Agaricus bisporus. The invention further relates to genes, nucleotide sequences or DNA fragments encoding a ripening form of such a hydropobin, as well as recombinant DNA material containing said gene, nucleotide sequence or DNA fragment. The invention further relates to a process for producing a ripening form of a hydrophobin, a protein or a polypeptide essentially corresponding thereto, derived from an edible fungus, comprising expression of such a gene, gene sequence or DNA fragment, for instance by culturing a cell containing this genetic material. In a further aspect, the invention relates to a regulatory region of a hydrophobin gene from an edible fungus, recombinant DNA material containing said region, as well as to a method for expressing genes in a fungus, preferably an edible fungus, in which said genes are brought to controlled expression under the control of said regulatory region. This method is preferably carried out using the genus Agaricus, especially the fruit bodies thereof.