METHOD OF RADIOMETRIC DETERMINATION OF PHOSPHORIBOSYLPYROPHOSPHATE IN BIOLOGICAL TISSUES

摘要

Usage: to determine the content of the bi- fosforibozilpirofosfata ol | egichoskih tissues. SUMMARY OF THE INVENTION: length determining content fosforibozilpirofosfata reacted enzymatic synthesis of labeled hypoxanthine inosine monophosphate in the presence and absence of the standard fosforibozilpirofosfata, inosine monophosphate precipitated as the lanthanum salt which was then dissolved in 0.03 M hydrochloric acid, mixed with scintillation fluid and formation rate measured therein pulses. The invention allows to increase the accuracy of the analysis and fosforibozilpirofosfata reduce the time required for its implementation by selectively isolating the reaction product and determining its radioactivity counting in a homogeneous system.