METHOD FOR THE IMMUNO-ENZYMATIC ASSAY OF VITAMIN B12 AND PREPARATION OF A SAMPLE FOR THE ASSAY

摘要

P Immunoenzymatic assay method for vitamin B12 and method of preparing a sample for this assay. BR/ To detect a biological species (vitamin B1 2) present in a test sample bound to endogenous binding proteins, a mineral base and a thiol are activated to denature the proteins and release the species A buffering agent and a sulfhydryl conversion agent are added to convert the remaining thiol groups into disulfide groups. Sequential rather than competitive binding of vitamin B1 2 is then performed with an excess of intrinsic factor, followed by binding of excess enzyme-labeled vitamin B1 2 to the rest of the intrinsic factor, and this factor is immobilized on solid phase. An optical determination of the level of enzymatic activity, solid phase or solution is then carried out. / P