An immobilized lipase is prepared by adsorbing lipase on a carrier that is capable of retaining 3% or more by weight of water after drying under a reduced pressure of from 0.01 to 1.0 Torr at 70° C. for 60 hours. A cation exchange resin is a preferred carrier and a weakly acidic cation exchange resin is most preferred. The lipase adsorbed on the carrier can be a freeze dried lipase and the resultant carrier- adsorbed lipase is dried. Lecithin may be adsorbed on the carrier as an enzyme activity enhancing agent. The immobilized lipase may also contain a dispersing agent. The immobilized lipase can be used for modifying fats and oils.
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