Method of detecting angle of optical rotation in solution having time- dependent concentration, detection apparatus therefor, and detector cell therefor

摘要

In a detection apparatus, a linearly polarized light beam polarized in a polarizer (62) is converted by being transmitted through a Pockels cell (60), driven by an alternative voltage, into an alternative linearly polarized light beam and a circularly polarized light beam, which is transmitted through a sample solution flowing through a flow cell (68). The transmitted light beam is separated into two polarized light components, and an intensity thereof is measured to obtain a signal proportional to an angle of rotation by the sample solution. A signal corresponding to an angle of rotation caused by an optical rotary power of the sample solution is obtained by subtracting the signal obtained during irradiation of the circularly polarized light from the signal obtained during irradiation of the linearly polarized light. The sample solution (18) is infused into an optical path of the flow cell (68) under a condition where mean velocity is 0.5 to 5 m/sec.