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Animal cell transformant, method for obtaining the transformant and method for producing desired foreign gene product by using the transformant
Animal cell transformant, method for obtaining the transformant and method for producing desired foreign gene product by using the transformant
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机译:动物细胞转化体,获得该转化体的方法和通过使用该转化体产生所需外源基因产物的方法
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摘要
By screening an animal cell transformant, which has been obtained by introducing into wild-type animal cells a wild-type DHFR gene and a structural gene which codes a desired useful substance, with a medium containing methotrexate (MTX) at a concentration of 50 M or lower, both genes can be amplified effectively within an MTX resistant strain and a stable amplified transformant can also be obtained. The screening may be repeated using an increasing concentration of MTX. It is also possible to obtain stably the desired foreign gene product in a high yield by using the thus-obtained amplified transformant.
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