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Purification of active and inactive/latent forms of plasminogen activator inhibitor-1
Purification of active and inactive/latent forms of plasminogen activator inhibitor-1
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机译:活性和非活性/潜在形式的纤溶酶原激活物抑制剂1的纯化
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摘要
A method of separating active and inactive/latent forms of PAI- 1 is disclosed comprising loading a sample containing a mixture of active and inactive/latent forms of PAI-1 to either an anion or cation exchange resin of 10-50 micron particle size and eluting active and inactive/latent forms of PAI-1 from the resin into separate fractions. Also disclosed is a method of extracting soluble E. coli-expressed recombinant PAI-1 from the lysed E. coli host cells in a buffered solution, wherein the ionic strength of the buffered solution is 8-25 millisiemens.
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