Quantitative modulo of indirect bilirubin and kitsuto null for fixed quantity

摘要

PURPOSE:To make exact quantitative determination of indirect bilirubin by converting direct bilirubin to biliverdin by bilirubin oxydase and bringing the remaining indirect bilirubin into reaction with a diazo reagent. CONSTITUTION:Bilirubin oxidase is added to a sample contg. the direct bilirubin and indirect bilirubin and is acted in a suitable buffer soln. to effect the reaction (1st reaction) by which the bilirubin is converted to the biliverdin. A diazonium salt as a diazo reagent and a reaction accelerator are then added thereto to effect the reaction (2nd reaction) in the buffer soln., by which a diazo dye is formed. The absorption of the colored reaction liquid is measured by absorption wavelength of the formed dye to make the quantitative determination of the indirect bilirubin. The indirect bilirubin is, therefore, exactly quantitatively determined by effecting the 1st reaction and the 2nd reaction.