NATURALLY OCCURRING G-CSF DERIVATIVE, ITS PRODUCTION AND DNA BASE SEQUENCE THAT ENCODES FOR AMINO ACID SEQUENCE OF NATURALLY OCCURRING G-CSF DERIVATIVE

摘要

Derivatives of naturally occurring G-CSF having at least one of the biological properties of naturally occurring G-CSF, and a solution stability of at least 35% at 5 mg/ml are disclosed in which the derivative has at least Cys17 of the native sequence replaced by a Ser17 residue and Asp27 of the native sequence replaced by a Ser27 residue. Nucleotide sequences coding for part or all of the amino acid sequence of the derivatives of the invention may be incorporated into autonomously replicating plasmid or viral vectors employed to transform or transfect suitable procaryotic or eucaryotic host cells such as bacteria, yeast or vertebrate cells in culture.