SIEVE TUBE PROTEIN GENE OF PLANT AND METHOD FOR MAKING USEFUL PROTEIN TRANSMIGRATE TO SIEVE TUBE USING THE SAME

摘要

PURPOSE: To make a useful protein transmigrate to a sieve tube by using a gene of a protein migrating to the sieve tube of a plant. ;CONSTITUTION: A sieve tube juice is collected from a rice plant by utilizing a planthopper. A protein contained in the sieve tube juice is separated by a secondary electrophoretic method to determine a partial amino acid sequence of a principal protein. A probe prepared based on the sequence is used to search for a cDNA library of a leaf of rice plant. The resultant gene of the sieve tube is fused to a gene of a trypsin inhibitor and transduced into the rice plant. Thereby, the fused protein is made to transmigrate to the sieve tube of the rice plant.;COPYRIGHT: (C)1994,JPO&Japio