6-ALKOXYMETHOXYMALTOOLIGOSIDE DERIVATIVE, ALPHA-AMYLASE ACTIVITY DETERMINATION REAGENT CONTAINING THE DERIVATIVE AS ACTIVE COMPONENT AND DETERMINATION OF ALPHA-AMYLASE ACTIVITY

摘要

PURPOSE: To provide a new derivative capable of easily and accurately determining amylase activity in a short time and useful as a reagent for the determination of amylase activity. ;CONSTITUTION: The derivative of formula I {(n) is 2-6; X is aromatic chromophore; Y¹ is CH(R¹)-O-R² or CH(R¹)-S-R² [R¹ is H or a (substituted) hydrocarbon group; R² is (substituted) hydrocarbon group or together with R¹ form an alkylene]; Y² is (substituted) hydrocarbon group, alkyl, etc.}, e.g. 2-chloro-4-nitrophenyl 4⁵,6⁵-di-O-(2-methoxy)ethoxymethyl-β-Dmaltopentaoside. The compound of formula I can be produced e.g. by reacting a compound of formula II with a compound of formula III (R²⁰ is methoxy, ethoxy, etc.; R²¹ is methoxy or ethoxy), etc., in an aprotic polar solvent (e.g. DMF) in the presence of p-toluenesulfonic acid as a catalyst, subjecting the resultant compound of formula IV to acylation and dealkoxymethylidenation reaction, alkoxymethylating the 6-OH group and finally introducing a substituent to the 4-OH group.;COPYRIGHT: (C)1994,JPO&Japio