Determination of the Active Component(s) of Heparin Associated with Wound Healing After Severe Burn: Structural Analysis and Biological Activity.

摘要

Heparin and casein are two potential burn healing compounds. Their effect on growth of mammalian cells cultivated in vitro has been investigated. Heparin appears to have some growth promoting effect on prepuce cells, while it showed little effect on growth of Novikoff hepatoma, monkey kidney and baby hamster kidney cells. Heparin also reversed the inhibitory effect of prednisolone-21-sodium-succinate of growth of prepuce and baby hamster kidney cells. Partially hydrolyzed casein having an amino N/total N ratio of 0.09 exhibited growth-stimulating activity for prepuce cells which resembles activity of newborn calf serum. This activity was abolished when casein was more fully hydrolyzed with trypsin. (Author)