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PREPARATION OF GIANT LIPOSOME, DEVICE FOR PRODUCING LB FILM, AND PRODUCITON OF LB FILM USING THE DEVICE
PREPARATION OF GIANT LIPOSOME, DEVICE FOR PRODUCING LB FILM, AND PRODUCITON OF LB FILM USING THE DEVICE
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机译:巨大的脂质体的制备,用于生产LP胶片的设备以及使用该设备生产LB胶片的设备
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摘要
PURPOSE: To provide a device for producing LB film with which giant liposomes having sufficient mechanical and physical strengths and excellent stability and having diameters of tens of micrometers or larger, especially hundreds of micrometers, can be produced in a large amount in a simple manner under mild conditions and in which LB film formation can be conducted simultaneously with any of various oxidation or reduction reactions; and to provide a method of producing an LB film using the device. ;CONSTITUTION: An aqueous alkali metal salt solution (a) which contains, dispersed therein, liposomes containing a polymerizable lipid is frozen and melted, and is then dialyzed against an aqueous salt solution or buffer which has a lower salt concentration than the solution (a). The obtained enlarged liposomes are maintained at a certain temp. to further coalesce the droplets into larger ones. The polymerizable lipid contained in the layers of the resulting giant liposomes is then polymerized. In LB film formation, an electrode is disposed either on the surface of a liquid phase for developing LB molecules or inside the phase and an electric current is applied so as to flow through the electrode and a substrate on which an LB film is to be deposited.;COPYRIGHT: (C)1994,JPO&Japio
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