thermo inducible expression of heterologous genes in bacillus subtilis and means and methods for its implementation.

• 1. 一株胞内表达枯草芽孢杆菌漆酶的基因工程菌株及实现该菌株分泌表达漆酶的诱导方法 [P] . 中国专利： CN104593312A . 2015-05-06
• 2. 适于酵母中的异源基因表达的启动子 [P] . 中国专利： CN106414744B . 2020.11.06
• 3. thermo inducible expression of heterologous genes in bacillus subtilis and means and methods for its implementation. [P] . 德国专利： DE69009883D1 . 1994-07-21

  机译： 枯草芽孢杆菌中异源基因的热诱导表达及其实施方法和方法。

• 4. The thermo-inducible expression of heterologous genes in bacillus subtilis and means and methods for its achievement [P] . 欧洲知识产权局专利： EP0410228B1 . 1994-06-15

  机译： 枯草芽孢杆菌中异源基因的热诱导表达及其实现方法

• 5. The thermo-inducible expression of heterologous genes in bacillus subtilis and means and methods for its achievement [P] . 欧洲知识产权局专利： EP0410228A1 . 1991-01-30

  机译： 枯草芽孢杆菌中异源基因的热诱导表达及其实现方法

• 1. Self-induced Secretion Expression of Trehalose Synthase in Bacillus subtilis WB800n [J] . Xihui WANG, Hongling LIU, Songsen SUI, 农业生物技术：英文版 . 2019,第002期
• 2. Cloning and Expression of Extremely Heat-Resistant Endo-β-1, 4-Glucanase Gene from Thermotoga maritima in Bacillus subtilis [J] . HAO Yarong, CHEN Ting, ZHANG Xingqun 东华大学学报（英文版） . 2019,第005期
• 3. Expression of a Lysine-rich Gene in Bacillus subtilis 168 [J] . Li Xue-lin, Liu Xiao-fei, Gao Xue-jun, 东北农业大学学报（英文版） . 2013,第001期
• 4. Novel expression of plectasin in Bacillus subtilis by a maltose inducible vector [C] . Licong Zhang, Anshan Shan World biomaterials congress . 2016
• 5. Cloning and expression of the novel glgC genes from Chlamydia trachomatis and Bacillus subtilis and directed evolution of the recombinant Chlamydia ADP-glucose pyrophosphorylase. [D] . Berger, Christopher Nicholas. 2005
• 6. A general method for the consecutive integration of single copies of a heterologous gene at multiple locations in the Bacillus subtilis chromosome by replacement recombination. [O] . J A Kiel, A M ten Berge, P Borger, 1995
• 7. Construction of the secretion vector system using Bacillus amyloliquefaciens neutral protease gene and expression of heterologous gene in Bacillus subtilis [O] . Terasaki Jun, テラサキ ジュン 1992
• 8. Cloning and Expression of the Bacillus anthracis Protective Antigen Gene in Bacillus subtilis. [R] . Ivins, B. E., Welkos, S. L. 1986

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Induced Transcriptional Expression of iBacillus subtilis/iAmino Acid Permease iyvbW/iin Response to Leucine Limitation [J] . Sean M. Rollins . 微生物学（英文） . 2014,第8期
• 3. 热纤梭菌Clostridium thermocellum的β-1,3-1,4-葡聚糖酶基因LicB在枯草芽孢杆菌Bacillus subtilis中的表达研究 [C] . 罗紫臣 ,鲍杰 . 纪念中国微生物学会成立六十周年大会暨2012年中国微生物学会学术年会 . 2012
• 4. Streptomyces lividans TK24 sec启动子表达特性的研究;异源基因在蛋白酶体缺失菌株中的分泌表达研究 [A] . 王丽非 . 2004