PROCEDURE FOR THE DETERMINATION OF OSTEOCALCINA IN PLASMA OR HUMAN SERUM.

摘要

FOR THE DETERMINATION OF OSCTEOCALCINE IN HUMAN SERUM OR PLASMA, A PROCEDURE IS INCLUDED IN WHICH A TEST OF BIOLOGICAL FLUID IS CONTAINED THAT CONTAINS THE OSTEOCALCINE TO BE DETERMINED TOGETHER WITH A KNOWN NUMBER OF AN ANO-ANCILLED LAYER OF ANO-ANCILLED APPLIANCE LIKE THE PLOTTER. THIS PROCEDURE HAS BEEN PROVEN TO BE WRONG, SINCE INCUBATION CONDITIONS INFLUENCE MEASURED VALUES AND VERY HIGH OSTEOCALCINE VALUES WERE OBTAINED. THE KNOWLEDGE THAT THE ERRORS OBSERVED ARE DUE TO A PROTEOLITIC DISINTEGRATION OF THE OLIGOPEPTIDE TRACKER THROUGH SUBSTANCES CONTAINED IN THE SERUM OR PLASMA, LEAD TO THE IDEA OF ADDING TO THE TEST OF HUMAN SERUM OR PLASMA ANT PLASMA OF OLIGOPEPTIDES ONE OR SEVERAL EMNDOPEPTIDASE INHIBITING SUBSTANCES WITH AN AMINOPEPTIDASE INHIBITING SUBSTANCE, OR, USING A PROTECTED N-TERMINAL OLIGOPEPTIDES TRACTOR, ONLY ONE INHIBITED SUBSTANCE. AMASTATINE AND LEUPEPTINE ARE PREFERABLE AS INHIBITING SUBSTANCES. DETERMINATION OF OSTEOCALCINE WITH DIAGNOSTIC VIEWS.