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Method and apparatus for cryopreparing biological tissue for ultrastructural analysis
Method and apparatus for cryopreparing biological tissue for ultrastructural analysis
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机译:超低温分析生物组织以进行超微结构分析的方法和装置
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摘要
This invention relates to a method for the cryopreparation of biological tissue samples for ultrastructural analysis. The process comprises vitrifying a biological tissue sample under severely rapid conditions and then depressurized. The depressurized, vitrified tissue sample is brought to equilibrium at a temperature of loss than -140° C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is infiltrated with a degassed resin and followed by a polymerization of the resin to form an embedded tissue sample. This invention additionally relates to a sample holder apparatus which has unique use characteristics in the process of this invention.
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