Preparing process of physiologically active substance with anticancer effect and substance obtained thereby

摘要

Preparing process of physiologically active substance with anticancer effect and the substance prepared by the process. This process comprises: transplanting of cancer cells in a mammal abdominal cavity; administering an anticancer agent in the abdominal cavity; intravenously administering spleen cells extracted from a normal and the same kind of mammal in a vena of the mammal to which cancerous cells have been transplanted; collecting ascites retained in the abdominal cavity after elapse of a predetermined time period; and fractionating a supernatant liquid of the ascites by Cohn's fractionation to isolate Cohn V fraction of said supernatants.