Membrane Type Wastewater Decolorization Using Culture Supernatant of Trametes versicolor and Squeezing of Physiologically Active Substance from Fungus Body

摘要

An enzymatic membrane bioreactor using the culture supernatant of white-rot fungus Trametes versicolor was developed to perform the decolorization of dye wastewater. The culture supernatant exhibiting high laccase activity and the model dye wastewater containing anthraquinone type dye were mixed in a continuous stirred tank membrane reactor with an ultrafiltration membrane which permitted the recycling of the enzyme in the reactor. The results obtained from the enzymatic membrane bioreactor showed that 80% of decolorization and approximately-constant permeation resistance were maintained for continuous treatment periods. The decolorization behaviors were well described using the combined equation on the basis of mass balance and enzyme kinetics. In addition, dehydration of excess fungus that grown in the process of the decolorization treatment was carried out by mechanical expression. It should be noted that the moisture content in the compressed cake of fungus was finally reduced to 20wt% under pressure of 7 MPa. The kinetics of expression such as the time variation of the moisture content in the compressed cake were accurately described on the basis of the multi-stage creep model. Furthermore, we confirmed that the squeezed liquid from the fungus body contains physiologically active substances such as (3-glucan showing antitumor activity.