STRAIN OF BACTERIUM BACILLUS PUMILIS PRODUCING RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5amp;prime;- CCTNAGC- 3amp;prime;

摘要

FIELD: biotechnology. SUBSTANCE: strain Bacillus pumilis VKPM B-5464 is detected which is a producer of restrictase Bpu101. This enzyme recognizes and cleaves nucleotide sequence 5′- CCTNAGC- 3′. Starin is isolated from the soil after restrictase producers screening. Strain grows on the simple, available and cheap medium. Yield of enzyme is 200 U/g wet biomass, specific activity of enzyme is 100 U/ml. Strain provides restrictase production showing unique site of recognition and cleaving of nucleotide sequence 5′- CCTNAGC- 3′. EFFECT: detection of bacterial strain indicated above. 1 dwg, 1 tbl