STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5amp;prime;-CC(A/T)-GG-3amp;prime;

摘要

FIELD: microbiology, biotechnology. SUBSTANCE: new strain of bacterium Bacillus stearothermophilus ВКПМ B-5465 is proposed a producer of restriction endonuclease which can recognize and cleave nucleotide sequence 5′-CC(A/T)-GG-3′. Strain is isolated from thermal water sources as a result of restrictase producer screening. The yield of enzyme is 100000 U/g wet biomass, specific activity of the end enzyme is 30000 U/ml. Restrictase Bst 381 is an isoschizomer of restrictase Bst GII and can replace the latter in all genetic engineering studies. EFFECT: isolation of enzyme indicated above, increased specific activity. 1 dwg