RECOMBINANT PLASMID DNA PCGA7 ENCODING SURFACE ANTIGEN OF HEPATITIS B VIRUS (HBSAG) AND YEAST STRAIN SACCHAROMYCES CEREVISIAE - A PRODUCER OF HEPATITIS B VIRUS SURFACE ANTIGEN

摘要

FIELD: biotechnology, medicine. SUBSTANCE: recombinant plasmid pCGA7 is reconstructed. Plasmid contains gene HBsAg under control 5′- and 3′-regulatory sequences of yeast gene PHO5. Yeast 1RDB 11/pCGA7 is obtained. Productivity HBaAg is 2 mg/1 l culture showing 100% plasmid stability under nonselective growth condition. EFFECT: enhanced yield of antigen HBaAg.