Bile acid sulfate - sulfatasegen, a plasmid which contains the gene, and a method for the production of bile acid sulfate - sulfatase

摘要

A method for the production of bile acid sulfate - sulfatase, which comprises the steps of:a. insertion of a dna - fragment, which is obtainable by a method, comprising the steps of:I. extracting from chromosomal dna from a pseudomonas testosteroni - strain with bile acid sulfate - sulfatase - activity andIi. digestion of the chromosomal dna with the restriction enzymes psti and smai, in order to at least one dna - fragment obtained, which has a size of 2,36 kb and a polynucleotide encoding a peptide of the following amino acid sequence (seq id no: 1) codes: in a vector - dna, in order to obtain a recombinant dna,example, transforming a escherichia coli - strain having of the recombinant dna,c. screening a transformant, the recombinant dna with the dna - fragment of 2,36 kb, by tes2in the bile acid sulfate - sulfatase - activity,d. culturing the transformant in a culture medium bile acid sulfate - sulfatase, and methode. recovery of bile acid sulfate - sulfatase from the culture.