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Positive selection vector for the bacteriophage P1 cloning system
Positive selection vector for the bacteriophage P1 cloning system
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机译:噬菌体P1克隆系统的阳性选择载体
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摘要
Positive selection cassettes are disclosed which contain a lethal gene, a promoter, a repressor sequence overlapping the promoter, and a cloning site between the promoter and the lethal gene. Insertion of a foreign nucleic acid sequence into the cloning site prevents expression of the lethal gene. Expression of the lethal gene under nonrepressed conditions kills a host organism containing a positive selection cassette which does not contain the foreign nucleic acid sequence.
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