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Assays for SOD activity due to the use of automatic oxidizable compounds, automatic oxidizable compounds and their preparation
Assays for SOD activity due to the use of automatic oxidizable compounds, automatic oxidizable compounds and their preparation
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机译:使用自动氧化性化合物,自动氧化性化合物及其制备方法测定SOD活性
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PCT No. PCT/FR92/01093 Sec. 371 Date Jun. 6, 1994 Sec. 102(e) Date Jun. 6, 1994 PCT Filed Nov. 25, 1992 PCT Pub. No. WO93/11258 PCT Pub. Date Jun. 10, 1993The method for assaying the SOD (super oxide dismutase) activity in liquid medium is based on the activation of self-oxidization, by SOD activity, of a reactive agent having the general formula (I) wherein either n is 1 or 2, R1 is -OR4 or -NR5R6; R2 is H, -OR4, alkyl (1-6C), -CH2- or -CH2-CH2-, to form a ring by binding to the phenyl substituent, at meta with respect to R1; and R3 is H, alkyl (1-6C) or -OR4 (if R2 is different from -OR4); with R4 being H or alkyl (1-6C); R5 being H, alkyl (1-6C), -CH2COOH, -C6H5COOH or -C6H5SO3H; and R6 is H, alkyl (1-6C) or -CH2COOH; or n is 1, R1 is -OR4, R2 is -CH2-O-, in order to form a ring by bonding of O with the phenyl substituent, at meta with respect to R1; and R3 is H or -OR4. Application to assaying the SOD activity in a sample, specially a biological sample, particularly by a single measurement and one calibrating curve.
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