Assays for SOD activity due to the use of automatic oxidizable compounds, automatic oxidizable compounds and their preparation

摘要

PCT No. PCT/FR92/01093 Sec. 371 Date Jun. 6, 1994 Sec. 102(e) Date Jun. 6, 1994 PCT Filed Nov. 25, 1992 PCT Pub. No. WO93/11258 PCT Pub. Date Jun. 10, 1993The method for assaying the SOD (super oxide dismutase) activity in liquid medium is based on the activation of self-oxidization, by SOD activity, of a reactive agent having the general formula (I) wherein either n is 1 or 2, R1 is -OR4 or -NR5R6; R2 is H, -OR4, alkyl (1-6C), -CH2- or -CH2-CH2-, to form a ring by binding to the phenyl substituent, at meta with respect to R1; and R3 is H, alkyl (1-6C) or -OR4 (if R2 is different from -OR4); with R4 being H or alkyl (1-6C); R5 being H, alkyl (1-6C), -CH2COOH, -C6H5COOH or -C6H5SO3H; and R6 is H, alkyl (1-6C) or -CH2COOH; or n is 1, R1 is -OR4, R2 is -CH2-O-, in order to form a ring by bonding of O with the phenyl substituent, at meta with respect to R1; and R3 is H or -OR4. Application to assaying the SOD activity in a sample, specially a biological sample, particularly by a single measurement and one calibrating curve.