METHODS FOR PRODUCING TRANSGENIC NON-HUMAN ANIMALS HARBORING A YEAST ARTIFICIAL CHROMOSOME

摘要

The present invention provides methods and compositions for transferring large transgene polynucleotides and unlinked selectable marker polynucleotides into eukaryotic cells by a novel method designated co-lipofection. The methods and compositions of the invention are used to produce novel transgenic non-human animals harboring large transgenes, such as a transgene comprising a human APP gene or human immunoglobulin gene.