Formation manner null of homogeneous polyploids

摘要

PURPOSE:To obtain the subject polyploid for producing industrially useful genetic recombinant at high frequency and in a short time without using genetic marker by exposing test cell of conidia or mycelium of fungi to colchicines having polyploid-organization potency in cell division period. CONSTITUTION:Test cell (e.g. Trichoderma reesei QM 9414 strain) of conidia or mycelium of fungi is subjected to shake culture in liquid medium containing colchicine or/and colchicine derivative having polyploid-organization potency (e.g. colcemid) in cell division period of said cell at a temperature of 30 deg.C for 2-3 day and the cell formed many pellet-like aggregates is transplanted on agar-agar medium, then cultured at a temperature of 30 deg.C for 2-3 day to afford the aimed autopolyploid of fungi having increased nuclear size.