SAMPLE PREPARING METHOD, BAND WIDTH MINIMIZING METHOD FOR SAMPLE ELUATE AND CHROMATOGRAPH ELUATE AND SAMPLE COMPONENT CONCENTRATING METHOD

摘要

PURPOSE: To concentrate an object to be analyzed on a column, minimize the band width and improve the analyzing efficiency and precision by mixing a surface active agent in a sample to be filled in the column and pouring moving phase to separate the object to be analyzed. ;CONSTITUTION: Surface active agent 107 reserved in a source 106 is mixed with sample 105 from a sample manipulator 104 to produce sample/surface active agent mixture 109. The mixture 109 is injected into a column 110 with a sample injector 116. On the other hand, a column pump 118 supplies moving phase solvent 120 in a reservoir 121 into the column 110. The composition of the solvent 120 is adjusted by a gradient programmer 122. Temperatures in the sample/surface active agent reservoir 108 and the column 110 are controlled by temperature controllers 126 and 128, respectively. The absorbance of sample components separated in elusion is detected by a detector 112 and the concentration of object to be analyzed is calculated from the absorbance of the component band. The peak of the enriched object to be analyzed brings about highly sensitive and precise detection.;COPYRIGHT: (C)1995,JPO