Promo expression of foreign genes in yeast - data - Louis, plasmids consisting, and the production of polypeptides

摘要

Promoters capable of ensuring expression in yeast of genes coding for heterologous polypeptides are disclosed, which comprise a DNA fragment or mutants or sub-fragments thereof, said promoter comprising a definite nucleotide sequence commencing with an EcoRI site and terminated by another restriction site. Also disclosed are vector plasmids containing the promoters, transformed yeasts comprising these plasmids, and a process for preparing polypeptides and especially a 1,4- beta -N-acetylmuramidase.