DNA PRIMER FOR AMPLIFICATION OF ANTIBODY GENE, PRIMER FOR SYNTHESIS OF CDNA CODING ANTIBODY MOLECULE AND VECTOR FOR PRODUCTION OF CHIMERA ANTIBODY

摘要

PURPOSE: To provide a new DNA primer useful for the most uniform amplification of an IgG variable region gene applicable to a variable region gene of chimera antigen. ;CONSTITUTION: The objective 3'-side DNA primer for the amplification of an antibody gene contains a base sequence containing a nicking site of a type-II restriction enzyme complimentary to a region having high preservability of the J region of IgG or contains a base sequence added with a nicking site of a type-II restriction enzyme complimentary to a region having high preservability of the J region of IgG. The 3'-side DNA primer has the base sequence of e.g. formula I or formula II when the antibody gene is human or mouse IgG K chain variable region gene. It can be synthesized usually by known oligonucleotide synthesis method according to the base sequence. The synthesis is preferably carried out by using a DNA synthesizer.;COPYRIGHT: (C)1995,JPO