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RIBOSOME-CYTOSKELETON BINDING FACTOR, PRODUCTION THEREOF AND METHOD FOR RECOVERING RIBOSOME USING
RIBOSOME-CYTOSKELETON BINDING FACTOR, PRODUCTION THEREOF AND METHOD FOR RECOVERING RIBOSOME USING
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机译:核糖体骨架结合因子,其生产及其使用核糖体的恢复方法
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摘要
PURPOSE: To provide a method for rapidly, simply and stably recovering a ribosome by which a ribosome-cytoskeleton binding factor is obtained; the operation is hardly restricted by a centrifuge and the application to any of micro- to macrosamples can be made. ;CONSTITUTION: This ribosome-cytoskeleton binding factor has about 30-150kDa molecular weight, an affinity for a heparin and further activities in sedimenting a ribosome and this method for producing the factor is provided. Furthermore, this method for recovering the ribosome comprises mixing a liquid containing the ribosome with the ribosome-cytoskeleton binding factor at a low ionic concentration, reacting both and precipitating the ribosome.;COPYRIGHT: (C)1995,JPO
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