POLYPEPTIDE HAVING AMINO ACID SEQUENCE RELATED TO ANTIHUMAN HIGHLY AFFINITIVE IGE RECEPTOR MONOCLONAL ANTIBODY AND DNA FRAGMENT CAPABLE OF CODING THE SAME

摘要

PURPOSE: To obtain the subject new polypeptide, capable of specifically recognizing a human highly affinitive IgE receptor, elucidating an antigenic region of an antihuman affinitive IgE receptor monoclonal antibody and useful for treating and diagnosing, etc., allergic inflammations, etc. ;CONSTITUTION: This polypeptide selected from formula I (FR1 to FR4 each is each a polypeptide residue; CDR1H to CDR3H each is an H chain variable region) and formula II (FR5 to FR8 each is a polypeptide residue; CDR1L to CDR3L each is an L chain variable region) is obtained by administering a human highly affinitive IgE receptor as an antigen to a Balb/c mouse, immunizing the mouse, collecting a cell of the spleen thereof, fusing the resultant cell to a cell of a myeloma, selectively culturing the fused cell, providing a hybridoma cell, cloning the cell, affording a hybridoma cell capable of producing an antihuman highly affinitive IgE receptor monoclonal antibody, subsequently isolating an mRNA from the resultant cell, synthesizing a cDNA using the mRNA as a template, amplifying the H and L chains in the variable region of a murine antibody according to a polymerase chain reaction (PCR) and expressing the amplified H and L chains with a host cell.;COPYRIGHT: (C)1995,JPO