A METHOD OF PRODUCING A POLYPEPTIDE PRODUCT AND A PLASMIDIC EXPRESSION VEHICLE THEREFOR, A METHOD OF CREATING AN EXPRESSION PLASMID, A METHOD OF CLEAVING DOUBLE STRANDED DNA, AND SPECIFIC PLASMIDS

摘要

Method for producing a heterologous polypeptide by bacterial expression of a structural Gene that encodes IT,Comprising (a) a bacterial Inoculant have transformed with a vehicle of expression Plasmid Replication with a sequence of double stranded DNAIn Phase from a First end to a Second end 5 '3' Strand of the coding for the sameElements: (i) a system of TRP Promoter Operator bacterial; (ii) nucleotides encoding for a place of Attachment of ribosomes for translation of the item (iv); (iii) nucleotides codificFor a start the signalfrom Translation Item (iv); and (iv) a structural Gene that encodes the amino acid sequence of a polypeptide heterologous; (b) placing the Inoculant is transformed intoA fermentation Container and make it grow, and (c) deprive bacteria desreprimir Additive System and cause the expression product to encode The Structural Gene.