PROCEDURE FOR OBTAINING SURFACE ANTIGENS FROM THE HEPATITIS B VIRUS WITH A GREATER IMMUNOGENIC CAPACITY AND ITS USE IN PREPARING A VACCINE.

摘要

This Invention belongs to the field of Genetic Engineering and the appropriate adjuvant is highly immunogenic in animals and humans.The Field of application of this result is mainly the production of recombinant vaccines against hepatitis B virus for use inAlthough the Human Antigen thus obtained can also be used for Diagnostic purposes.Biotechnology and in particular the production of vaccines against hepatitis B virus.The Fundamental objective of the same is to obtain high levels of surface antigen of hepatitis B virus with properAda particulacion and high degree of Purity for use in vaccine preparation with immunogenic Capacity increased with a view to their utilizacIon in Human.To achieve this goal is a procedure whose First Step consisted in the creation of a suitable host vector System iThrough a genetic Construct that contains the Gene of the surface antigen of hepatitis B virus in an expression cassetteWhich was used for the transformation of a Mutant Strain of P. pastoris his - called MP 36.The obtained recombinant Strain,C 226 is grown under conditions that allow a High Production of recombinant Antigen and soluble form.Intracellular and particles, with a size of 22 nm.Subsequently, it has a process of Extraction and purification to achieveThrough the use of a monoclonal antibody designed to this end, a product of high purity and homogeneity which when coupled !