Methods Purifiacion involving Mimetic Affinity Reagent aminoacidocomo grinding.

摘要

We describe a method to isolate a Protein from a Sample,Including (i) to provide a First Molecule which is capable of forming a complex with the Protein Affinity; (ii) contacting the sample with one Molecule under conditions that allowFormation of Complex deafinidad; (iii) to isolate the complex; (iv) to treat the complex with a Second Molecule.The Second Molecule, mimicking the Protein amino acid residue or the first Molecule that is critical for the formation decomplejo, so the second Molecule breaks the complexCreate the fragmentation release of Protein Complex; and (V) to isolate the Protein.According to a form of realization, is used as a Mimetic amidazola deaminou00e1cido leaching Reagent very soft to break thecomplex Protein and Antibody fusion protein.A tu00e9cnicaque has general application to the isolation of Antibodies oproteu00ednas fusion Antibody, recombinant.