YEAST MUTANTS WITH DEFECTS IN N-GLYCOSYLATION AND THEIR USE IN EXPRESSING UNIFORMLY GLYCOSYLATED PROTEINS

摘要

Saccharomyces mutants with defects in N-glycosylation which are obtainable by [3H]-mannose suicide selection, introduction of one or more selective markers, selection of those strains which, after transformation with the plasmid YEpL/GOD, on cultivation under standard conditions secrete 10 mg/l GOD or more into the culture medium, are allelic to the ngd mutations contained in Saccharomyces cerevisiae DSM 7042, DSM 7338, DSM 7160 and/or 7340, and express proteins with a uniform carbohydrate structure.