Physiological and isotonic buffer compatible with atp-luciferase bioluminescence reaction

摘要

A novel formulation is provided for a buffer which uses sugars instead of salts, to provide an isotonic environment which is osmotically balanced with living cells but causes minimal inhibition of the ATP-luciferin-luciferase bioluminescence reaction. The buffer also nourishes and sustains the cells as shown by prolonged survival in the buffer without cell lysis. The buffer was developped for use in a sperm viability test based on intracellular ATP determination, but is suitable for use in viability assays including tissue cultured cells, homopoietic and other tissue derived cellular suspensions.