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PHYSIOLOGICAL AND ISOTONIC BUFFER COMPATIBLE WITH ATP-LUCIFERASE BIOLUMINESCENCE REACTION
PHYSIOLOGICAL AND ISOTONIC BUFFER COMPATIBLE WITH ATP-LUCIFERASE BIOLUMINESCENCE REACTION
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机译:与ATP-尿嘧啶酶生物发光反应兼容的生理和等渗缓冲液
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摘要
A novel formulation is provided for a buffer which uses sugars instead of salts, to provide an isotonic environment which is osmotically balanced with living cells but causes minimal inhibition of the ATP-luciferin-luciferase bioluminescence reaction. The buffer also nourishes and sustains the cells as shown by prolonged survival in the buffer without cell lysis. The buffer was developped for use in a sperm viability test based on intracellular ATP determination, but is suitable for use in viability assays including tissue cultured cells, homopoietic and other tissue derived cellular suspensions.
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