Recombinant staphylococcal nucleases and their use in limiting the survival of genetically engineered microorganisms

摘要

The survival of cells can be limited by introducing into the cells a regulatably expressible gene whose expression results in the formation of a cytoplasmatically active truncated and/or mutated Staphylococcus aureus nuclease. Populations of cells containing, in addition to such a regulatably expressible gene, a DNA coding for an immunologically active, pesticidally active or environmental pollutant-degrading gene product are useful in immunologically active compositions, pesticidally active compositions and environmental pollutant-degrading compositions, respectively.