HIGH THROUGH-PUT QUANTITATIVE POLYMERASE CHAIN REACTION FOR HIV CLINICAL SPECIMENS

摘要

Methods and kits for the rapid and accurate quantitation of HIV-1 nucleic acids in large numbers of human clinical samples is disclosed. Cloned, standard internal controls for both HIV-1 and cellular sequences are used for parallel amplification and compared with the test samples. A universal HIV-1 gag primer permits detection and quantitation of sequences from every known HIV-1 gag gene while liquid hybridization detection greatly reduces processing time and error.