RECOMBINANT ANTIBODY FRAGMENTS WHICH ARE SYNTHESIZED AND BIOTINYLATED IN E. COLI, THEIR USE IN IMMUNOASSAYS AND IMMUNOPURIFICATION TECHNIQUES

摘要

Production of recombinant antibody fragments Fab expressed in E. Coli, wherein the Fd portion is biotinylated in vivo following the fusion of the coding region of the antibody with that of a biotine receptor. The invention also concerns the utilisation of the bifunctional character of said biotinylated antibody fragments for the production of immunoassays and for developing antigen immunopurification methods.